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❶The PCR process contains these three steps: I still love quizzes.

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The PCR Process

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The advent of the polymerase chain reaction (PCR) radically transformed biological science from the time it was first discovered (Mullis, ). For the first time, it allowed for specific detection and production of large amounts of DNA.

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The ELISPOT assay is a highly sensitive, relatively easy-to-perform, and reproducible method for identification and quantification of even very rare antigen-specific T cells.

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Collective cell migration is a hallmark of wound repair, cancer invasion and metastasis, immune responses, angiogenesis, and embryonic morphogenesis. These include transcriptome analysis with RNA sequencing (RNA-seq) (Antonini et al., x Antonini et al., Antonini, D., Mollo, M.R., and Missero, C. Research techniques made simple: identification and characterization of long noncoding RNA in dermatological research.

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The questions and answers below relate to the Research Techniques Made Simple article “Enzyme Immunoassay (EIA) and Enzyme-Linked Immunosorbent Assay (ELISA),” published online with the September issue of JID. Correct answers appear in bold . RNA sequencing is a method of transcriptome profiling that utilizes next-generation sequencing technology. It offers several distinct advantages over hybridization-based approaches, most notably superior sensitivity and the capacity for de novo transcript discovery. This article describes RNA sequencing methodology, summarizes important technological advances and challenges, and .